Head coach Steve Borthwick has named a 36-player squad for the tournament, assembling at Pennyhill Park on Monday. In the absence of Mitchell, Bath scrum-half Ben Spencer and Bristol Bears' Harry Randall have been called into the setup as scrum-half options alongside Leicester Tigers player Jack van Poortvliet.

Mitchell sustained the injury during Northampton's 28-14 win at Franklin's Gardens, leaving the pitch after just 21 minutes. The 29-year-old has established himself as the first-choice number nine under Borthwick, making his prolonged absence a significant blow to England's preparations.

Compounding the scrum-half shortage, uncapped Northampton player Archie McParland is also sidelined after sustaining a calf injury during training with the national squad last week. Consequently, Borthwick faces a major reshuffle in the half-back department ahead of Sunday's opening fixture against the Wallabies.

Van Poortvliet, who started all three of England's summer Tests, is widely expected to wear the starting shirt at Allianz Stadium. Both Randall and Spencer offer contrasting strengths from the bench, with Randall renowned for his sniping running game and Spencer valued for his tactical kicking.

In other squad news, fly-half Fin Smith took a limited part in training upon arrival as he continues his recovery from a calf problem. England coaching staff remain optimistic about his availability for Sunday's encounter, though Harlequins playmaker Marcus Smith is also pushing for the starting fly-half berth.

Several other players are managing injuries within the 36-man group, including hooker Luke Cowan-Dickie and flanker Tom Curry. Both participated in modified training sessions at the Bagshot base but are expected to be fit for selection by the end of the week.

Borthwick's squad features 20 forwards and 16 backs as England return to Allianz Stadium for four consecutive Test matches. Following Sunday's clash with Australia in round four of the competition, England will host Japan in round five and New Zealand in round six.

The autumn series concludes with the finals weekend scheduled from November 27 to 29. Borthwick highlighted the importance of the upcoming matches, noting they provide a final opportunity to face southern hemisphere opposition before next year's Rugby World Cup.

"We're looking forward to getting back to Allianz Stadium for four important Test matches against top-level opposition," Borthwick said. "These fixtures take on added significance this autumn as they represent our final opportunity to face southern hemisphere opposition before next year's World Cup."

England squad: Forwards: Fin Baxter, Ollie Chessum, Arthur Clark, Alex Coles, Luke Cowan-Dickie, Ben Curry, Tom Curry, Theo Dan, Ben Earl, Greg Fisilau, Jamie George, Ellis Genge, Joe Heyes, Maro Itoje, Emmanuel Iyogun, George Martin, Asher Opoku-Fordjour, Tom Pearson, Henry Pollock, Will Stuart. Backs: Seb Atkinson, Fraser Dingwall, Immanuel Feyi-Waboso, George Ford, Tommy Freeman, George Furbank, Will Joseph, Joe Marchant, Cadan Murley, Harry Randall, Henry Slade, Fin Smith, Marcus Smith, Ben Spencer, Freddie Steward, Jack van Poortvliet.